CELL PROLIFERATION ANALYSIS

BrdU incorporation can be used to assess the proliferation status of tissues of
transgenic mice. Embryos in utero can be labeled by injection of BrdU into pregnant
female mice; postnatal animals can be labeled by direct intraperitoneal injection of
BrdU. The quantity of BrdU and the labeling time must be tailored to the tissue of
interest. We provide a protocol below that works well for craniofacial tissues