It is often important to know the sites and levels of expression of the transgene.
Hence the detection of the messenger RNA or the protein (or both) encoded by the
transgene may be useful. In addition, if the transgene is likely to alter cell fate, then
an analysis of molecular markers is likely to provide critical information on embryonic
territories and the differentiation status of cells composing an affected structure. For
these purposes—transgene expression and molecular marker analysis—both whole
mount immunohistochemistry and in situ hybridization are extremely useful
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