More detailed analysis of phenotypes entails histological methods. We generally
use standard paraformaldehyde fixation followed by paraffin embedding (described in
detail in Subheading . It is sometimes useful to embed in plastic (historesin),
which provides excellent tissue preservation and also enables lacZ to be visualized
under dark field with great sensitivity . Standard H and E stain usually serves well.
Bright-field or phase-contrast microscopy are generally adequate for visualization of
craniofacial structures, although differential interference contrast (DIC) optics provide
exceptionally good views of developing calvarial bone and the osteoblastic cells that
compose the osteogenic front (unpublished observations).
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